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Unlike in Navigate to... feature where the route is calculated from start to end by a routing service, Navigation along a route navigates recorded tracks, imported or planned routes stored in your own Locus track/route folders.
Locus Map automatically generates navigation commands along the route. If you need to be navigated in reverse direction, make a copy of the route and check both the “Change track orientation” and “Generate commands” options in the copy dialog.
If you want to be navigated strictly along the selected route (e.g. on a bike), check “Strict route navigation” and un-check “Automatic recalculation” in the navigation settings.